Equipment

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Microscopes

  • FEI Tecnai T12 – 120 kV TEM equipped with LaB6 filament and a Gatan Ultrascan 2k X 2k CCD.
  • FEI Talos Arctica  – 200 kV cryo-TEM equipped with a 6k x 4k Gatan K3 direct electron detector and running SerialEM package for automated data collection.
  • FEI Titan Krios –  300 kV cryo-TEM microscope equipped with a Gatan K3 6k x 4k direct electron detector, Gatan Energy Filter, and phase plates. The 3 condenser lens system of this microscope, along with the Fringe Free Illumination, allows for a wide range of beam settings while maintaining parallel illumination. Single particle and tomography data collection is automated with SerialEM.

Ancillary equipment

  • Plunge Freezers  FEI Vitrobot (Mark IV x2, )
  • Glow Discharge  Pelco easiGlow, Emitech K100X
  • Carbon Coating  Cressington 208carbon
  • High Pressure Freezing  BalTec HPM010 high-pressure freezer system for bulk tissue and cell vitrification. Leica AFS automated freeze substitution processor
  • Serial Sectioning  Cryo and conventional ultramicrotome (Leica EM UC6/FC6)

External Equipment

  • FEI Versa with Quorum Cryo stage  SEM with a focused ion beam for sample screening and lamellar preparation located in Caltech’s Kavli Nanoscience Institute
  • Cryo Light Microscopy Linkam cryo stage on a Zeiss LSM 880 Airyscan confocal light microscope located in Caltech’s Biological Imaging Facility