Acknowledgement

Please provide the following statement in any publications that used center resources:

“(Cryo) Electron microscopy was performed in the Beckman Institute Resource Center for Transmission Electron Microscopy at Caltech. “

Publications

2019

    1. Escolano A*, Gristick HB*, Abernathy ME, Merkenschlager J, Gautam R, Oliveira TY, Pai J, West AP Jr, Barnes CO, Cohen AA, Wang H, Golijanin J, Yost D, Keeffe JR, Wang Z, Zhao P, Yao KH, Bauer J, Nogueira L, Gao H, Voll AV, Montefiori DC, Seaman MS, Gazumyan A, Silva M, McGuire AT, Stamatatos L, Irvine DJ, Wells L, Martin MA, Bjorkman PJ, Nussenzweig MC. Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques. Nature, 2019. DOI: 10.1038/s41586-019-1250-z [Epub ahead of print]
    2. Schoofs T*, Barnes CO*, Suh-Toma N, Golijanin J, Schommers P, Gruell H, West AP Jr, Bach F, Lee YE, Nogueira L, Georgiev IS, Bailer RT, Czartoski J, Mascola JR, Seaman MS, McElrath MJ, Doria-Rose NA, Klein F, Nussenzweig MC, Bjorkman PJ. Broad and Potent Neutralizing Antibodies Recognize the Silent Face of the HIV Envelope. Immunity 50 (6), 2019. DOI: 10.1016/j.immuni.2019.04.014
    3. Jones C, Asay M, Kim L J, Kleinsasser J, Saha A, Fulton T, Berkley K, Cascio D, Malyutin A, Conley M, Stoltz B, Jose V, Nelson R H. Characterization of reactive organometallic species via MicroED. ChemRxiv 2019. DOI: 10.26434/chemrxiv.7940525.v2.
    4. Kaplan M, Subramanian P, Ghosal D, Oikonomou CM, Pirbadian S, Starwalt-Lee R, Mageswaran SK, Ortega DR, Gralnick JA, El-Naggar MY, Jensen G. In situ imaging of the bacterial flagellar motor disassembly and assembly processes. EMBO 38, 2019. DOI: 10.15252/embj.2018100957.
    5. Farhadi A, Ho GH, Sawyer DP, Bourdeau RW, Shapiro MG. Ultrasound imaging of gene expression in mammalian cells. Submitted. bioRxiv 2019. DOI: 10.1101/580647
    6. Ghosal D*, Jeong KC*, Chang Y-W, Gyore J, Teng L, Gardner A, Vogel JP, Jensen G. Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS. Nature Microbiology 2019. DOI: 10.1038/s41564-019-0427-4
    7. Qing Y, Sara J. W,Jee-Young M, Grant J. J. Fusion of DARPin to Aldolase Enables Visualization of Small Protein by Cryo-EM. Structure 27, 1–8, 2019. DOI: 10.1016/j.str.2019.04.003
    8. Ladinsky MS, Araujo LP, Zhang X, Veltri J, Galan-Diez M, Soualhi S, Lee C, Irie K, Pinker EY, Narushima S, Bandyopadhyay S, Nagayama M, Elhenawy W, Coombes BK, Ferraris RP, Honda K, Iliev ID, Gao N, Bjorkman PJ, Ivanov II. Endocytosis of commensal antigens by intestinal epithelial cells regulates mucosal T cell homeostasis. Science 363, 2019. DOI: 10.1126/eaat4042
    9. Uchil PD, Pi R, Haugh KA, Ladinsky MS, Ventura JD, Barrett BS, Santiago ML, Bjorkman PJ, Kassiotis G, Sewald X, Mothes W. A protective role for the Lectin CD169/Siglec-1 against a pathogenic murine retrovirus. Cell Host and Microbe 25, 87-100, 2019. DOI: 10.1016/j.chom.2018.11.011
    10. Azubel M, Carter SD, Weiszmann J, Zhang J, Jensen GJ, Li Y, Kornberg RD. FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles. eLife 2019. DOI: 10.7554/eLife.43146
    11. Ghosal D, Kim KW, Zheng H, Kaplan M, Vogel JP, Cianciotto NP, Jensen G. In vivo structure of the Legionella type II secretion system by electron cryotomography. bioRxiv 2019. DOI: 10.1101/525063
    12. Martynowycz MW, Zhao W, Hattne J, Jensen G, Gonen T. Collection of continuous rotation MicroED data from ion beam-milled crystals of any size. Structure 27 (3), 545-548, 2019. DOI: 10.1016/j.str.2018.12.003
    13. Kaplan M, Ghosal D, Subramanian P, Oikonomou CM, Kjaer A, Pirbadian S, Ortega DR, Briegel A, El-Naggar MY, Jensen G. The presence and absence of periplasmic rings in bacterial flagellar motors correlates with stator type. eLife 2019. DOI: 10.7554/eLife.43487
    14. Chreifi G, Chen S, Metskas LA, Kaplan M, Jensen G. Rapid tilt-series acquisition for electron cryotomography. J Struct Bio 205 (2), 163-169, 2019. DOI: 10.1016/j.jsb.2018.12.008

2018

    1. Bourdeau RW, Lee-Gosselin A, Lakshmanan A, Farhadi A, Ravindra Kumar S, Nety ST, Shapiro MG. Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature 553, 86-90, 2018. Cover article. DOI: 10.1038/nature25021
    2. Wang H*, Barnes CO*, Yang Z, Nussenzweig MC, Bjorkman PJ. Partially-open HIV-1 envelope structures exhibit conformational changes relevant for coreceptor binding and fusion. Cell Host and Microbe 24, 579-592, 2018. DOI: 10.1016/j.chom.2018.09.003
    3. Donaldson GP, Ladinsky MS, Yu KB, Sanders JG, Yoo BB, Chou WC, Conner ME, Earl AM, Knight R, Bjorkman PJ, Mazmanian SK. Gut microbiota utilize immunoglobulin A for mucosal colonization. Science 360, 795-800, 2018. DOI: 10.1126/science.aaq0926
    4. Yao Q*, Weaver SJ*, Mock J-Y, Jensen G. Fusion of DARPin to aldolase enables visualization of small protein by cryoEM. bioRxiv 2018. DOI: 10.1101/455063
    5. Kaplan M, Subramanian P, Ghosal D, Oikonomou CM, Pirbadian S, Starwalt-Lee R, Gralnick JA, El-Naggar MY, Jensen G. Stable sub-complexes observed in situ suggest a modular assembly pathway of the bacterial flagellar motor. bioRxiv 2018. DOI: 10.1101/369405
    6. Ghosal D, Chang Y-W, Jeong KC, Vogel JP, Jensen G. Molecular architecture of the Legionella Dot/Icm type IV secretion system. bioRxiv 2018. DOI: 10.1101/312009, 2018.
    7. Chang Y-W, Shaffer C, Rettberg L, Ghosal D, Jensen G. In vivo structures of the Helicobacter pylori cag type IV secretion system. Cell Reports 23 (3), 673-681, 2018. DOI: 10.1016/j.celrep.2018.03.085
    8. Subramanian P, Pirbadian S, El-Naggar M, Jensen G. Ultrastructure of Shewanella oneidensis MR-1 nanowires revealed by electron cryotomography. PNAS 115 (14), E3246-E3255, 2018. DOI: 10.1073/pnas.1718810115
    9. Auer G, Oliver P, Rajendram M, Yao Q, Jensen G, Weibel D. Bacterial swarming reduces Proteus mirabilis and Vibrio parahaemolyticus cell stiffness and increases β-lactam susceptibility. bioRxiv 2018. DOI: 10.1101/275941
    10. Krause D, Chen S, Shi J, Jensen A, Sheppard E, Jensen G. Electron cryotomography of Mycoplasma pneumoniae mutants correlates terminal organelle architectural features and function. Molecular Microbiology 108 (3), 306-318, 2018. DOI: 10.1111/mmi.13937
    11. Swulius M, Mguyen L, Ladisnky M, Ortega D, Aich S, Mishra M, Jensen G. Structure of the fission yeast actomyosin ring during constriction. PNAS 201711218, 2018. DOI: 10.1073/pnas.1711218115

2017

    1. Lakshmanan A, Lu GJ, Farhadi A, Nety SP, Kunth M, Lee-Gosselin A, Maresca D, Bourdeau RW, Yin M, Yan J, Witte C, Foster FS, Schroder L, Shapiro MG*. Preparation of biogenic gas vesicle nanostructures for use as contrast agents for ultrasound and MRI. Nature Protocols 12, 2050-2080, 2017. DOI: 10.1038/nprot.2017.081
    2. Carter S, Mageswaran S, Farino Z, Mamede J, Oikonomou C, Hope T, Freyberg Z, Jensen G. Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells. Journal of Structural Biology 201 (1), 15-25, 2017. DOI: 10.1016/j.jsb.2017.10.009
    3. Gristick, HB, Wang, H, Bjorkman, PJ. X-ray and EM structures of a natively glycosylated HIV-1 envelope trimer. Acta Cryst. D 73: 822–828, 2017. DOI: 10.1107/S2059798317013353
    4. Kieffer, C, Ladinsky MS, Bjorkman, PJ. Multiscale imaging of HIV-1 transmission in humanized mice. AIDS Res. Hum. Retroviruses 33(S1):S6-S7, 2017. DOI: 10.1089/AID.2017.0142
    5. Wang, H, Gristick, HB, Scharf, L, West, AP, Jr, Galimidi, RP, Seaman, MS, Freund, NT, Nussenzweig, MC, Bjorkman, PJ. Asymmetric recognition of HIV-1 Env trimer by V1V2 loop-targeting antibodies. eLife 6, e27389, 2017. DOI: 10.7554/eLife.27389.001
    6. Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ, Nikolova L, Bjorkman PJ, Sundquist WI, Ullman KS, Frost A. LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells. PNAS USA 114, E2166-E2175, 2017. DOI: 10.1073/pnas.1613916114
    7. Meier E, Daitch A, Yao Q, Bhargava A, Jensen G, Goley E. FtsEX-mediated regulation of the final stages of cell division reveals morphogenetic plasticity in Caulobacter crescentus. PLoS Genetics 13(9), e1006999. DOI: 10.1371/journal.pgen.1006999
    8. Kieffer C, Ladinsky MS, Ninh A, Galimidi RP, Bjorkman PJ. Longitudinal imaging of HIV-1 spread in humanized mice with parallel 3D immunofluorescence and electron tomography. eLife 6, e23282, 2017. DOI: 10.7554/eLife.23282.001
    9. Freund, NT, Wang, H, Scharf, L, Nogueira, L, Horwitz, JA, Bar-On, Y, Golijanin, J, Sievers, SA, Sok, D, Cai, H, Cesar Lorenzi, JC, Halper-Stromberg, A, Toth, I, Piechocka-Trocha, A, Gristick, HB, van Gils, MJ, Sanders, RW, Wang, L-X, Seaman, MS, Burton, DR, Gazumyan, A, Walker, BD, West, AP, Bjorkman, PJ, Nussenzweig, MC. Coexistence of potent HIV-1 broadly neutralizing antibodies and antibody-sensitive viruses in a viremic controller. Sci Transl Med 9, eaal2144, 2017. DOI: 10.1126/scitranslmed.aal2144
    10. Zielinska A, Billini M, Moll A, Kremer K, Briegel A, Martinez A, Jensen G, Thanbichler M. LytM factors affect the recruitment of autolysins to the cell division site in Caulobacter crescentus. Molecular Microbiology 106 (3), 419-438, 2017. DOI: 10.1111/mmi.13775
    11. Kaplan M, Yoo B, Tang J, Karam T, Liao B, Majumdar B, Baltimore D, Jensen G, Zewail A. Photon-induced near-field electron microscopy (PINEM) of eukaryotic cells. Angewandte Chemie International Edition 56 (38), 11498-11501, 2017. DOI: 10.1002/anie.201706120
    12. Briegel A, Oikonomou C, Chang Y-W, Kjaer A, Huang A, Kim K, Ghosal D, Nguyen H, Kenny D, Ogorzalek Loo R, Gunsalus R, Jensen G. Morphology of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis. EMBO Reports e201744070, 2017. DOI: 10.15252/embr.201744070
    13. Jeong K, Ghosal D, Chang Y-W, Jensen G, Vogel J. Polar delivery of Legionella type IV secretion system substrates is essential for virulence. PNAS 201621438, 2017. DOI: 10.1073/pnas.1621438114
    14. Dobro MJ, Oikonomou CM, Piper A, Cohen J, Guo K, Jensen T, Tadayon J, Donermeyer J, Park Y, Solis BA, Kjaer A, Jewett AI, McDowall AW, Chen S, Chang Y-W, Shi J, Subramanian P, Iancu CV, Li Z, Briegel A, Tocheva EI, Pilhofer M, Jensen GJ. Uncharacterized bacterial structures revealed by electron cryotomography. J.Bacteriology 2017.  DOI: 10.1128/JB.00100-17