Equipment

FEI Tecnai T12 – 120 kV TEM equipped with LaB6 filament and a Gatan Ultrascan 2k X 2k CCD.

FEI Talos Arctica  – 200 kV cryo-TEM equipped with a 6k x 4k Gatan K3 direct electron detector and running SerialEM package for automated data collection.

FEI Titan Krios –  300 kV cryo-TEM microscope equipped with a Gatan K3 6k x 4k direct electron detector, Gatan BioQuantum Energy Filter, and phase plates. The 3 condenser lens system of this microscope, along with the Fringe Free Illumination, allows for a wide range of beam settings while maintaining parallel illumination. Single particle and tomography data collection is automated with SerialEM

Thermo Scientific Cryo Hydra Dual Beam – coming 2025.

Plunge Freezers  FEI Vitrobot (Mark IV x2), Leica EM GP2

Glow Discharge  Pelco easiGlow, Emitech K100X

Carbon Coating  Cressington 208carbon

High Pressure Freezing  Wohlwend Compact-03 high-pressure freezer system for bulk tissue and cell vitrification. Leica AFS automated freeze substitution processor. Please contact Mark for the process.

Serial Sectioning  Cryo and conventional ultramicrotome (Leica EM UC6/FC6). Please contact Mark to get sections.

FEI Versa with Quorum Cryo stage  SEM with a focused ion beam for sample screening and lamellar preparation located in Caltech's Kavli Nanoscience Institute

Cryo Light Microscopy Linkam cryo stage on a Zeiss LSM 880 Airyscan confocal light microscope located in Caltech's Biological Imaging Facility