The Center offers the following training resources
- Simons Electron Microscopy Center at the New York Structural Biology Center cryo-EM overview
- What Could Go Wrong? A Practical Guide to Single-Particle Cryo-EM: From Biochemistry to Atomic Models – a review by Michael A. Cianfrocco and Elizabeth H. Kellogg
- Prof. Jensen's online videos on the theory and principles of cryo-EM (14 hours)
- Prof. Jensen and Dr. Matthijn Vos' online videos demonstrating plunge-freezing, clipping, loading, and safety (4 hours)
- Prof. Jensen and Dr. Matthijn Vos' online videos on the theory and operation of cryo-EMs and single particle data collection (~40 hours) This resource will soon offer multiple-choice self-tests. PI's may choose to establish minimum performance standards for their students before moving on in the training program.
Other online resources:
Individual PIs may require their lab members to demonstrate mastery of online materials before hands-on training
Standard Operating Procedure (SOP) documents that are written and maintained by managing directors are available next to each instrument to remind and guide users, and on the website's Caltech-access-only knowledge base.
After taking maximum advantage of the above resources, if possible, those who wish to learn how to prepare samples and/or operate microscopes on their own should first try to shadow experienced users who can sign up for microscope time independently. The direct training will be one-on-one with the Managing Director. The training progress will be based on the trainee's experience and individual progression, with the direct supervision gradually reduced as the trainee's progress permits. Following a demonstration of proficiency by the trainee, the Managing Director may designate them as independent users. Please contact email@example.com to schedule direct training.