Training Outline
Training Resources
NIH sponsored curriculum
- Grant Jensen's "Getting Started in cryoEM" course - Caltech
- Peter Shen and Janet Iwasa's "cryoEM 101" - Univ. of Utah
- Yingjie Chen and Weng Jiang's "cryo VR" - Purdue
- Fred Sigworth's "Principles of cryoEM" - Yale
Other online resources
- PNCC Training page
- NCCAT Remote Learning Central
- S2C2 CryoEM Training Curriculum
- What Could Go Wrong? A Practical Guide to Single-Particle Cryo-EM: From Biochemistry to Atomic Models – a review by Michael A. Cianfrocco and Elizabeth H. Kellogg
SOPs
Standard Operating Procedure (SOP) documents that are written and maintained by managing directors are available next to each instrument to remind and guide users, and on the website's Caltech-access-only knowledge base.
Direct Training
After taking maximum advantage of the above resources, if possible, those who wish to learn how to prepare samples and/or operate microscopes on their own should first try to shadow experienced users who can sign up for microscope time independently. The direct training will be one-on-one with the Managing Director. The training progress will be based on the trainee's experience and individual progression, with the direct supervision gradually reduced as the trainee's progress permits. Following a demonstration of proficiency by the trainee, the Managing Director may designate them as independent users. Please contact [email protected] to schedule direct training.