Training Outline
The Center offers the following training resources
Online Tutorials
- Simons Electron Microscopy Center at the New York Structural Biology Center cryo-EM overview
- What Could Go Wrong? A Practical Guide to Single-Particle Cryo-EM: From Biochemistry to Atomic Models – a review by Michael A. Cianfrocco and Elizabeth H. Kellogg
- Prof. Jensen's online videos on the theory and principles of cryo-EM (14 hours)
- Prof. Jensen and Dr. Matthijn Vos' online videos demonstrating plunge-freezing, clipping, loading, and safety (4 hours)
- Prof. Jensen and Dr. Matthijn Vos' online videos on the theory and operation of cryo-EMs and single particle data collection (~40 hours) This resource will soon offer multiple-choice self-tests. PI's may choose to establish minimum performance standards for their students before moving on in the training program.
Other online resources:
Individual PIs may require their lab members to demonstrate mastery of online materials before hands-on training
SOPs
Standard Operating Procedure (SOP) documents that are written and maintained by managing directors are available next to each instrument to remind and guide users, and on the website's Caltech-access-only knowledge base.
Direct Training
After taking maximum advantage of the above resources, if possible, those who wish to learn how to prepare samples and/or operate microscopes on their own should first try to shadow experienced users who can sign up for microscope time independently. The direct training will be one-on-one with the Managing Director. The training progress will be based on the trainee's experience and individual progression, with the direct supervision gradually reduced as the trainee's progress permits. Following a demonstration of proficiency by the trainee, the Managing Director may designate them as independent users. Please contact cryoem@caltech.edu to schedule direct training.